dCas9-ω

dCas9 fused to the RNA polymerase omega subunit; recruits RNAP to activate a weak promoter (bacterial CRISPRa).

dCas9 / Cas variantCRISPRaactivate

Details

Type
dCas9 / Cas variant
Hosts
E. coli, broad-host
CRISPR action
activate
PAM / PFS
NGG (PAM)

Used in designs

Source

Reference
Bikard et al. 2013, NAR