In-vitro CRISPR-Dx designer
Design a nucleic-acid detection assay — DETECTR (Cas12a), SHERLOCK (Cas13a/d), or a dCas9-binding sensor. Paste a pathogen target (or load a legacy example), and get ranked detection crRNAs plus a matched RPA pre-amplification primer pair — complementing the whole-cell library.
1 Platform & target
DETECTR · LbCas12a · targets dsDNA · TTTV PAM (5prime) · needs pre-amplification (RPA). Targets dsDNA; on recognition, collateral ssDNase activity cleaves an FQ reporter. Typically preceded by isothermal pre-amplification (RPA).