dCas9-VPR

dCas9 fused to the VPR tripartite activator; strong CRISPRa in eukaryotes (yeast, mammalian).

dCas9 / Cas variantCRISPRaeukaryotic

Details

Type: dCas9 / Cas variant
Hosts: yeast, mammalian
CRISPR action: activate
PAM / PFS: NGG (PAM)

Used in designs

Androgen sensor (AR + CRISPRa)Copper sensor (ACE1 + CRISPRa)Estrogen / endocrine-disruptor sensor (ERα + CRISPRa)

Source

Reference: Chavez et al. 2015, Nat Methods