SpCas9 sgRNA scaffold
Standard S. pyogenes single-guide RNA scaffold; append the 20-nt spacer at the 5' end.
sgRNA scaffoldsgRNAscaffold
Details
- Type
- sgRNA scaffold
- Hosts
- broad-host
Sequence
GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGC
Used in designs
Not referenced by a library design yet — available in the designer.
Source
- Reference
- Qi et al. 2013, Cell